Author: mpolin

SynBio Teaser Week: how it happened (take 2)

Photo 25-09-2014 09 50 40Today wePhoto 25-09-2014 10 47 18 had the second microfluidics half-day for the SynBio students. We really enjoyed our time with them, and hope they enjoyed it too. Thanks to our “training” yesterday, we were able to speed up things a bit, with the result that the students managed to extract tracks of swimming microalgae from their movies (this is a step further than just recognising the position of microorganisms in individual frames).

Photo 25-09-2014 12 06 35Three extra guests joined the students: Munehiro Asally, a newlyPhoto 25-09-2014 12 06 30 recruited Assistant Professor in Life Sciences here at Warwick; Paul MacDonald, a new Ph.D. student of Alfonso Jaramillo; and Christopher de Wolf, a technician/researcher from Orkun Soyer’s lab.

SynBio Teaser Week: how it happened

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Today, we hosted the first half of this year’s Photo 24-09-2014 10 18 35cohort of new Ph.D. student from the SynBio CDT for a chat about microfluidics. The students had a varied background (physics, chemistry, biology) but were all equally enthusiastic! We started with a presentation on microfluidics by Raphael, before heading down to the wetlab Photo 24-09-2014 10 34 46where we saw how to cut and bond a PDMS microfluidic channel onto a glass coverslip. Then the students took turns to cut a piece of glass capillary onto a microscope slide and filled it with a suspension of swimming algae (Chlamydomonas).

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The group then moved to the microscopy room to observe the algae and record a movie of their motion. Finally, they tried to Photo 24-09-2014 12 50 22make the computer recognise the position of the microorganisms in their movies using a particle tracking Matlab code. They actually managed to do it quite well! Well done everybody!!

Eukaryotic flagella synchronise through hydrodynamics

Two cells synch

Article published on eLife. See also accompanying Insight by B. Friederich and I. Riedel-Kruse.

After many years working on Chlamydomonas, we finally graduated to multiple cells… But from a different organism: Volvox carteri. This multicellular relative of Chlamy has thousands of biflagellate somatic cells on its surface, which can be easily extracted from the colony and keep on beating for several hours. We grabbed two with independent micropipettes and showed that below a critical separation, the cells synchronise their beating. Synchronisation has a purely hydrodynamic origin. At the same time, their interaction changes the waveform of their flagella. Flagellar elasticity cooperates with hydrodynamic stresses to generate synchrony as predicted a few years ago!